Objective: A simple, precise, accurate and specific high performance thin layer chromatographic method has been developed for the simultaneous estimation of zaltoprofen and paracetamol and Quantitation in pharmaceutical dosage form.
Methods: The method employed silica gel 60F254 Precoated plates as stationary phase and a mixture of chloroform: acetone: toluene: acetic acid (6.5 :2.5:1:0.1v/v/v/v) as mobile phase.
Results: The developed method was validated for accuracy, linearity, precision, specificity, limit of detection and limit of quantitation as per ICH guideline. The Rf value for ZLT and PCM were found to be 0.63±0.2 and 0.36±0.2 respectively. The calibration curve was found to be linear between 100-500 ng/spot for ZLT and 406.25-2031.25 ng/spot for PCM. The limit of detection and limit of quantitation for ZLT were found to be 13.45 and 40.78 ng/spot and for PCM 16.33 and 49.55 ng/spot respectively.
Conclusion: The proposed method has been successfully applied for the estimation of Zaltoprofen and Paracetamol in their pharmaceutical formulation.