ArticleSMALL SCALE LABORATORY CULTURE METHOD PRODUCTION AND CHARACTERIZATION OF L-GLUTAMIC ACID BY E.COLI
V. Asha , G. Tulja Rani, N.L.Mohammed , A. Mahipal, Ch. Naresh
L Glutamic acid is one of the major amino acid produced by microbial fermentation provides 90% of world’s total demand, and remaining 10% is met through chemical methods. It is the principal excitatory neurotransmitter in the brain and an important intermediate in metabolism. Present paper discuss the small scale laboratory culture method for production of glutamic acid. It was carried out by submerged fermentation technique where three different production media was prepared, sterilized, inoculated with the 24 hrs freshly prepared culture of E.coli. Production media was optimized with the selection of suitable carbon , nitrogen, minerals, vitamins, dyes which were required for the development of bacterial cells. Production medium was adjusted to pH7.0 with 1N HCl or 1N NaOH, kept in an orbital incubator shaker at 30°C at 120 rpm for 48 hr. End of the fermentation the cells and debris were removed by centrifugation at 10,000 rpm for 10 mts and supernatant solution was subjected to filtration. The filtrate was collected and dried to get the dry powder of glutamic acid. Progressive results and maximum yield of L- glutamic acid obtained with medium three and it was conformed with both chemical and analytical tests. L-glutamic acid produced by medium three showed positive results for ninhydrin and proved conformed result with xanthoproteic Test, UV spectroscopy and IR spectroscopy. This method can be applied in daily laboratory intended applications and these studies can be further extended for development of industrial techniques.
L Glutamic acid , E.COLI, fermentation, centrifugation